3) Isolation Technique : Cultivation Of Microbes

3) Isolation Technique :

Isolation of bacteria/microbes can be done after completion of incubation process carried out successfully here isolation means separating Pure Culture or specific targeted colony by help of picking up isolated single colony which is obtained on solid agar by the process including 

Inoculation → Incubation → Isolation with the help of strile inoculating loop.

The isolation is end result of inoculation and incubation after this isolation is the third main basic technique in from the 5 I concept of cultivation of Micro-organisms which we discuss earlier before in beginning of the article.

This process also known as "Isolation of Pure Culture".

To isolate the pure culture or colony after obtained growth on solid agar medium then the single well isolated tiny colony is selected for the isolation of pure culture selection where in liquid media its shows turbidity which means growth is obtained or incubation is conplete allow us to isolate the colony with the help of loop or taking 0.1 ml sample help of pipette simply Micro-pipette.

          Broth Medium Showing Turbidity

Isolated microbes can take separate colony (descrete mound of cell) on solid media.while turbidity is (free floating cells) in broth further this yield can be distinct colonies solid media known as "Sub-Culturing".

This process growth from isolated colony and inoculate further introduce into separate medium this one can take to pure culture contains single species of microbes.

This was all about the isolation if you are more interested you can visit link below 👇

https://conceptofmicrobiology.blogspot.com/2020/11/isolation-of-micro-organisms-by.html?m=1

     Isolation Microbe from broth Agar Plate

                 Sub-Culture Process