24) Lab Essentials : Nichrome Wire Loop / inoculating Loop
Microbiology Lab Essentials :
24) Inoculating Loop Or Nichrome Wireloop :
Consists of a looped nichrome wire fixed into a brass handle.
It is easy to sterilize and reuse because nichrome wire resists deterioration with repeated heat/cooling cycles.
Flame sterilization is a very simple method of killing microorganisms on an inoculating loop.
inoculating loop A tool usually made of platinum or nichrome wire in which the tip forms a small loop with a diameter of about 5 mm, and is used to smear, streak, or take an inoculum from, a culture of microorganisms.
Never lay the loop down once it is sterilised, or it may again become contaminated. Allow the loop to cool a few seconds before contacting the inoculum to avoid killing the microorganisms.
Courtesy : FlinnScientific
https://youtu.be/9k-DZWuTl_k
It can also beused to transfer microscopic organisms.
Touching a broth or a culture plate will gather enough microbes for inoculation.
Flame sterilization is a very quick simple method of killing microorganisms on an inoculating loop or needle.
The loop or needle is held inside a flame for a few seconds to bring it to redness and then cooled.
Once cool, the loop or needle can be used for various culture manipulations.
Courtesy : Microbiology Society -
https://youtu.be/-3AX6MYGqms
Platinum Inoculating Loops are used for bacteriological and other microbiological applications.
They will better resist constant exposure to acid and flame than the nichrome wire inoculating loop.
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