Kinetics Of Solute Uptake in Absence of Transporter :

Growth Kinetics :
2] Uptake in Absence of Transporter :
The Slow growing mutants which have been isolate that have no functional transporter for a particular nutrient.
In the absence of transporter the rate of solute entry is slow and does not approach a maximum rate even at very high concentration of solute (s), but it is proportional to the concentration gradient.

》Energy dependent transport -
Transporters facilitates the entry and exit the solute across the membrane.
At equilibrium it does not tends the transport in any direction and therefore causes accumulation of solute against a concentration gradient.
Many transport mechanisms catalyze the accumulation of solute into the cell.
The internal concentration when the steady state is reached can be several orders of magnitude higher than external concentration this requires energy.

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