Microbial Nutritions :
Microbial Nutritions :
The all living things need energy to perform functions and this form energy can be utilized by very very specific and at opportunistic levels here we are going to know about the nutritional requirement of Micro-organism.
Nutrients are the Substances which are used in biosynthesis & energy releasing activities performed by microorganism thats why they are required to play vital role for the growth of microorganisms.
This is grown by one of our follower Yaw Agyei Gyasi Lab from Africa Sample 'e.coli'
A Dry Cell Weights 95% Contains a few major elements : Carbon, Oxygen, Hydrogen, Nitrogen, Sulphur, Phosphorus, Potassium, Calcium, Magnesium, and Iron.
First six natural elements are
Carbon. (C)
Oxygen. (O)
Hydrogen. (H)
Nitrogen. (N)
Sulphur. (S)
Phosphorous. (P)
They are primitive components of (Precusors) of
1)Carbohydrates : These are the sugars, starch and fibers found in fruits,grains,vegetables & milk prod.
2)Lipids : Lipids are molecules that contain hydrocarbons and make up the building blocks of the structure and function of living cells.
3)Proteins : Proteins are essential nutrients they are one of the building blocks of body tissue and can also serve as a fuel source. As a fuel, proteins provide as much energy density as carbohydrates doimportant aspectprotein from a nutritional stand point is its amino acid composition.
The Microbial Nutritions Splits in to
1)Macro Nutrients
2)Micro Nutrients
We Will learn First
1)Macro Nutrients :
Carbon. (C)
Oxygen. (O)
Hydrogen. (H)
Nitrogen. (N)
Sulphur. (S)
Phosphorous. (P)
These Referred as Macroelements or Macronutrients because they are required in large amounts. The first six (C, O, H, N, S, and P) are components of carbohydrates, lipids, proteins, and nucleic acids.
The other Four Elements are
in the cell as cations and play a various roles
As Follows :
✓Potassium (K+) is required for activity by a number of enzymes, including some involved in protein synthesis.
✓Calcium (Ca 2+ ), among other functions, contributes to the heat resistance of bacterial endospores.
✓Magnesium (Mg 2+ ) serves as a cofactor for many enzymes, complexes with ATP, and stabilizes ribosomes and cell membranes.
✓Iron/Ferrous (Fe 2+ and Fe 3+ ) is a part of some molecules involved in the synthesis of ATP by electron transport-related processes (ETS ) or Electron Transport Chain System (ETCS).
These all elements are required for microorganisms in a huge amount.
Colors shows the various activity and utilization of Nutrients from agar-agar
2)Micro Nutrients : All microorganisms requires several nutrients in small amounts—amounts so small that in the lab they are often obtained as contaminants in water, glassware, and growth media.
Also available in nature, they are ubiquitous and usually present in adequate amounts to support the growth of microbes these nutrients are called Micronutrients or Trace Elements.
These Micronutrients Manganese (Mn),
Zinc (Zn), Cobalt (Co), Molybdenum (Mo),
Nickel (Ni), and Copper (Cu) these are needed by most cells.
Micronutrients are normally a part of enzymes and Co-factors, and they aid in the catalysis of reactions and maintenance of protein structure.
For example, Zinc (Zn 2+ ) is present at the active site of some enzymes but can also be involved in the association of different subunits of a multimeric protein.
Manganese (Mn 2+ ) aids many enzymes that catalyze the transfer of phosphate.
Molybdenum (Mo 2+ ) is required for Nitrogen (N2) fixation, and Cobalt (Co 2+ ) is a component of vitamin B12
These are Involved in Uptake of Nutrition by Microbes further topics will also on nutritional Requirement and Composition of Media.
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