Introduction to Cultivation Techniques in Microbiology : 👇

●Microbiological Cultivation Techniques :

✓Plating Techniques :

Microorganisms are present on all inanimate surfaces creating ubiquitous sources of possible contamination in the laboratory. Experimental success relies on the ability of a scientist to sterilize work surfaces and equipment as well as prevent contact of sterile instruments and solutions with non-sterile surfaces. Here we present the steps for several plating methods routinely used in the laboratory to isolate, propagate, or enumerate microorganisms such as bacteria and phage. All five methods incorporate aseptic technique, or procedures that maintain the sterility of experimental materials.

Note : It is recommended that non-pathogenic strains be used when learning the various plating methods.

✓Plating Techniques :
Why they are For ?
(1) streak-plating bacterial cultures to isolate single colonies,
(2) pour-plating and
(3) spread-plating to enumerate viable bacterial colonies,
(4) soft agar overlays to isolate phage and enumerate plaques, and
(5) replica-plating to transfer cells from one plate to another in an identical spatial pattern.
(1,2 & 3 are mostly used and Commonest Tecnhiques teach in Lab)
#Note : It is recommended that non-pathogenic strains be used when learning the various plating methods.

● Perform plating procedures without contaminating media. 

● Isolate single bacterial colonies by the streak-plating method. 

● Use pour-plating and spread-plating methods to determine the concentration of bacteria. 

● Perform soft agar overlays when working with phage. 

● Transfer bacterial cells from one plate to another using the replica-plating procedure.

A Short Safety Intro :

Be familiar with all laboratory rules and safety precautions to be taken when working with microorganisms. Regardless of biohazard classification, all materials that come in contact with microorganisms are considered infectious waste and must be decontaminated prior to disposal. Follow safety guidelines in accordance with those provided by institutional Environmental Health and Safety departments, setting up appropriate waste receptacles for immediate and proper disposal of potentially contaminated materials (biohazards).

✓The Experimenting Table :

•Safety and Precautions Must have things :

}Sterilize all instruments, solutions, and media prior to using them for plating procedures.

}Clear away all materials cluttering your work area on the laboratory bench.

}Clean work area with disinfectant to minimize possible contamination.

}Set up a Bunsen burner and work slowly, carefully, and deliberately within the sterile field area created by the updraft of the flame.

}Arrange all the supplies needed for the procedure on the laboratory bench near the sterile field. Make sure all the materials are properly labeled.

Place the Bunsen burner to your right on the bench.

Place agar plates or Petri dishes to your left.

Arrange cell cultures, tubes, flasks and bottles in the center of the bench.

Loosen the caps of tubes, flasks and bottles so they can be easily opened with one hand during subsequent manipulations.

Wash hands thoroughly with antiseptic soap and warm water before handling microorganisms.

Here is Something...

Dont Forget ...

To Organise your Table before Start....